Venom Blog

2006-08-28T16:22:00.000-07:00

-7:15-9:45: Meeting with Dr. Perez, Mark and Juan Carlos
9:45-10:00: Called companies to address payment issues on procurement card.
11:00-3:00 pm: Worked on protocol for excising venom glands used in molecular cloning. Wrote letter of recommendation for Gloria Chapa.
3:35-4:15 pm: Dealt with Burt Edmond from Beckman on setting up the Allegra 64 centrifuge. Spoke with Jacob on the phone regarding statistical issue on the crotatroxin paper. Spoke to a student who wants to volunteer at the NTRC.
4:15-5:30 pm: Finished protocol and e-mailed it to Dr. Perez for review.
5:30-6:00 pm: Made modifications on Crotatroxin paper.

**Keep your heart open to dreams. For as long as there's a dream, there is hope, and as long as there is hope, there is joy in living. ----Anonymous

**No Dream Comes True Until You Wake Up And Go To Work--Anonymous

2006-08-27T14:37:00.000-07:00

1:00-4:00 pm: Worked on Crotaroxin paper.
4:00-? Worked on Hemostatis Grant

2006-08-25T15:08:00.000-07:00

7:15-9:30 am: Met with Dr. Perez
9:30-10:45 am: Worked on B. colombiensis paper
10:45-11:00 am: Met with Mark on inputting HPLC profles on the database.
11:00-11:45 am: Dr. Mary Ann McLane called to discuss and correct crotatroxin paper.
1:00-3:00 pm: Worked on the corrections of crotatroxin paper.
3:45-4:45 pm: Met with Lisa and Esteban regarding issues with Sonoclot and 2D fractions.

**Keep your heart open to dreams. For as long as there's a dream, there is hope, and as long as there is hope, there is joy in living. ----Anonymous

2006-08-24T12:04:00.000-07:00

7:30-8:45 am: Performed and LD50 with Crotalus atrox venom.
8:45-9:15 am: Prepared Naja pallida venom for international shipment.
9:15-9:45 am: Ordered supplies.
9:45-12:00 pm: Worked on Crotatroxin paper and sent if off to Dr. McLane.
1:15 pm-1:30 pm: Met with a Biology student looking for research experience.

**Keep your heart open to dreams. For as long as there's a dream, there is hope, and as long as there is hope, there is joy in living. ----Anonymous

Back in the Blog of Things

2006-08-23T13:01:00.000-07:00

(Brasil-Photo by Elda)
7:30 am-9:00 am: Met with Dr. Perez
9:00-10:00 am: Ordered supplies since Nora is on vacation.
10:00-10:20 am: Met with Sharmin in accounting to transfer funds for purchasing equipment.
10:20-10:30 am: Order more supplies.
10:30-11:00 am: Worked on B. colombiensis paper
11:00-11:55 am: Went to Wal-Mart to buy portable oven for baking crucibles for molecular biology
1:00-1:17 pm: Checked e-mail and sent off e-mails
1:17-2:30 pm: Assisted in extracting venom from ~30 snakes. Mark Hockmueller was doing the extraction today.
2:30-3:15 pm: Reviewed Renato's mRNA extraction protocol.
4:00-5:30 pm: Worked on B. colombiensis paper.

**Keep your heart open to dreams. For as long as there's a dream, there is hope, and as long as there is hope, there is joy in living. ----Anonymous

Aug. 14, 2006

2006-08-15T01:28:00.000-07:00

8-8:15 am-Met with Lisa, Meli, Esteban and Gonz regarding assay results with PF2D fractions.
8:15-9:30-Met with Nora and Dr. Perez regarding NTRC issues.
9:30-12:30-Met with Dr. Perez regarding time and effort.
1:30-2:30-Logged into University Chase Account to input my purchases.
2:30-3:15-Worked on Crotatroxin paper.
3:45-4:14- Met with Dr. Martinez regarding the 1st sequence of Crotatroxin 1 to be used in publication. The sequence for Crotatroxin 2 will be finished by Friday.
4:15-5:30- Spoke with Dr. Julio Soto regarding the last paper submitted. Revisions will be made according to reviewers comments. Dr. Soto spoke about the assays that will be performed with the 11 mutants of Mojastin.
5:30-6:00-Spoke with Jacob regarding crotatroxin paper and his results of whole venom analysis by mass spectrometry.
9:00pm-3:30 am- Worked on Crotatroxin paper.

**Keep your heart open to dreams. For as long as there's a dream, there is hope, and as long as there is hope, there is joy in living. ----Anonymous

No Blogs Until Aug. 14

2006-08-05T10:18:00.000-07:00

Photo by Elda
Out of the center until Aug. 14.

**Keep your heart open to dreams. For as long as there's a dream, there is hope, and as long as there is hope, there is joy in living. ----Anonymous

Friday August 04

2006-08-05T05:55:00.000-07:00

-Gathered all NTRC publications for Arts and Science.
-Met with Lisa regarding her research.
-Weighed out 6 different venoms for Andrew.
-A bottle of acetic acid and gluteraldehyde broke in front of biology. Called Chris Tweddle and area was blocked off until it was cleaned.
-E-mailed Dr. Knutson on the dates for the seminar speakers. She will be out next week also, so we will resolve the week after. Forward the e-mails to Dr. Perez, Nora and Mark.
-E-mailed Dr. Tao regarding his September seminar. Forward info to Dr. Perez, Nora and Mark.
-E-mailed Tony Ramirez regarding the Honorable Juan M. Escobar's visit. Forward info to Dr. Perez, Nora and Mark.
-Ordered ECIS arrays for Rene.
-Attended graduation at 4:00. Gonzalo Lopez and Steven Reyes graduated.

**Keep your heart open to dreams. For as long as there's a dream, there is hope, and as long as there is hope, there is joy in living. ----Anonymous

Thurday August 03-Lost the Blog

2006-08-05T05:47:00.000-07:00

I had posted my blog for Thursday, but the system had some error and now it appears to be lost.

Anyways-I met with Dr. Perez from 7:30-9:00 regarding reports for clients. How can we improve them, file them and route them through proper channels.

9:00 -9:45 Dr. Perez and I met with Luis regarding Western blots.

9:50-10:15 Dr. Perez and I met with Gonzalo on his employment status.

-Met with Lisa regarding IEF gels on Micrurus fulvius fulvius venom.

-Ordered Reagents for Luis.
-Met with McNairs Scholar's Director, Myra Torres on paper work completion of the students.

-5:00-6:30 Met with Dr. Perez regarding different approach on training researchers.

**Keep your heart open to dreams. For as long as there's a dream, there is hope, and as long as there is hope, there is joy in living. ----Anonymous

2006-08-02T14:57:00.000-07:00

-Met with Lisa regarding IEF gels and HPLC runs.
-Had a meeting with Dr. Perez, Nora and Mark regarding pending projects.
-Prepared thrombin cleavage and purification of GST fusion protein bound to Glutathione Sepharose 4B.
-Extracted venom from 25 snakes.
-Sent Dr. Perez information on the reports sent to different clients.
-E-mailed Dr. Knutson on prefered seminar speakers.
-Ordered Thrombin from SIGMA
-E-mailed Midwest photos
-Met with Meli on PF2D Proteome.
-Isolated rMojastin from beads A280 reading 0.335-YEAH-Now I hope it has activity!!

**Keep your heart open to dreams. For as long as there's a dream, there is hope, and as long as there is hope, there is joy in living. ----Anonymous

2006-08-01T10:44:00.000-07:00

-Came in at 6:00 am and worked on Crotatroxin paper.

-Had a meeting with Dr. Perez from 7:00am to 9:00 am regarding the direction of the NTRC

-Went to Special Programs to see if all paper work for our students had been turned in.

-Went to the Serpentarium to get some venom for Lisa and Esteban's project.

**Keep your heart open to dreams. For as long as there's a dream, there is hope, and as long as there is hope, there is joy in living. ----Anonymous

2006-07-31T14:27:00.000-07:00

-Came in at 6:30 am and started washing beads for protein fusion.
-Had a brief meeting with Dr. Perez, Nora and Mark about not having a meeting until the afternoon.
-Met with Lisa on her research progress.
-Continued washing beads. Avanti 30 broke down again and had to rescue my samples by removing front panel of centrifuge.
-Went to the serpentarium to get venom for Javier, Meli and Lisa.
-Met with the artist who carved the mesquite snake. We went out to lunch with him.
-Continued bead washing.
-Met with Amy Tigerina on her research project. Looked for articles so she can incorporate in her paper.

2006-07-29T13:01:00.000-07:00

-Worked on B. colombiensis paper.

-Waited for artist who carved the mesquite snake.

**Keep your heart open to dreams. For as long as there's a dream, there is hope, and as long as there is hope, there is joy in living. ----Anonymous

2006-07-28T08:51:00.000-07:00

-Met with Dr. Perez, Nora and Mark regarding lab issues.

-Took broken camera lens to Mr. Diaz DeLeon so that he could fix it.

-Cleaned up molecular biology room.

-Sent letter off the Dr. Knutson regading seminar speakers from UT Health Science Center

-Met with Mike Lystiris of BECKMAN Coulter regarding the order on the MDQ. Walked him over to purchasing to meet with Bruce Payne.

-Attended a CEQ seminar given by Beckman Coulter.

**Keep your heart open to dreams. For as long as there's a dream, there is hope, and as long as there is hope, there is joy in living. ----Anonymous

2006-07-27T20:18:00.000-07:00

-Worked on the B. colombiensis paper.

-Walked over to Biology with safety officer to view some radioactive material that needed identifying.

-Trained Lisa, Esteban and Andrew on how to do an IEF gel on the PhastSystem.

-French Pressed some bacterial cells for over 4 hr to obtain the the disintegrin protein rMojastin 1. The cells were then treated with TritonX and centrifuged and stored in the -80˚C freezer. The French Press was taken apart to clean the piston chamber.

-Helped Shruti rescue her samples from the Avanti 30. The front panel had to be removed to activate the door. I guess we will be needing the other centrifuge that we purchased.

-Created my blog.

**Keep your heart open to dreams. For as long as there's a dream, there is hope, and as long as there is hope, there is joy in living. ----Anonymous

2006-07-27T20:11:00.000-07:00

ELDA ELIZA SáNCHEZ, Ph.D.
Assistant Director
Natural Toxins Research Center
Texas A&M University-Kingsville
Kingsville, TX 78363

**Keep your heart open to dreams. For as long as there's a dream, there is hope, and as long as there is hope, there is joy in living. ----Anonymous